SimBoolNet is an open source Cytoscape plugin for simulating the dynamics of signaling transduction using Boolean networks. In a Boolean network, every node is assigned a state of ON/OFF, and the state of a node is determined by the states of its upâstream neighboring nodes (i.e. nodes that are connected by an incoming edge). Given userâspecified levels of stimulation to a signaling network, SimBoolNet simulates the responses of the downstream molecules. The process can be visualized with an animation and the results recorded for further analysis. SimBoolNet is a useful tool for hypothesizing causal relation and crosstalk among signaling molecules.